Vibrio Culture | Orange County California

TEST NAME	Vibrio Culture
DEPARTMENT	BACT
TESTS	B27
DESCRIPTION	Screening procedure for the isolation and identification of Vibrio sp. utilizing conventional biochemical, serological, and matrix-assisted laser desorption ionization time of resolution (MALDI-TOF) techniques.
SPECIMEN REQUIREMENTS	SPECIMEN: Fresh stool CONTAINER: Stool transport bottles (Para-Pak C&S), GN Broth or Rectal Swabs Note: Buffered glycerol saline is unacceptable. The use of rectal swabs (Bacterial Culturettes) should be limited to patients with active disease, infants and children from whom feces may be difficult to obtain. Urine specimens must be processed within 4 hours if refrigerated (2-8°C) or transport in BD tubes within 96 hours. COLLECTION: The specimen of choice is the diarrheal stool collected during the acute stage of the disease. Keep the stool specimen cool, do not incubate or refrigerate. Portions containing blood or mucus usually contain the highest number of pathogens. TRANSPORT CONDITIONS: Room Temperature (25±5°C): 4 days (Preserved stool) Refrigerated (5±3°C): Not recommended Frozen (-15±5°C): Unacceptable CIDT Screens: 7 days
TURNAROUND TIME (TAT)	1 week
REFERENCE RANGE	Negative
TEST METHOD	Culture
CPT CODES	87077

TEST NAME

Vibrio Culture

DEPARTMENT

BACT

TESTS

B27

DESCRIPTION

Screening procedure for the isolation and identification of Vibrio sp. utilizing conventional biochemical, serological, and matrix-assisted laser desorption ionization time of resolution (MALDI-TOF) techniques.

SPECIMEN REQUIREMENTS

SPECIMEN: Fresh stool

CONTAINER: Stool transport bottles (Para-Pak C&S), GN Broth or Rectal Swabs
Note: Buffered glycerol saline is unacceptable. The use of rectal swabs (Bacterial Culturettes) should be limited to patients with active disease, infants and children from whom feces may be difficult to obtain. Urine specimens must be processed within 4 hours if refrigerated (2-8°C) or transport in BD tubes within 96 hours.

COLLECTION: The specimen of choice is the diarrheal stool collected during the acute stage of the disease. Keep the stool specimen cool, do not incubate or refrigerate. Portions containing blood or mucus usually contain the highest number of pathogens.

TRANSPORT CONDITIONS: Room Temperature (25±5°C): 4 days (Preserved stool)

Refrigerated (5±3°C): Not recommended

Frozen (-15±5°C): Unacceptable

CIDT Screens: 7 days

TURNAROUND TIME (TAT)

1 week

REFERENCE RANGE

Negative

TEST METHOD

Culture

CPT CODES

87077

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