Content block block-1354745981-1774396559
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| TEST NAME |
Syphilis Darkfield, Microscopic Exam |
|---|---|
| DEPARTMENT | BACT |
| TESTS | B22 |
| DESCRIPTION | Darkfield microscopy is used to demonstrate the presence of Treponema pallidum in lesions or aspirates in early-stage syphilis. |
| SPECIMEN REQUIREMENTS |
SPECIMEN: Serous fluid from genital lesion CONTAINER: Glass slide with coverslip COLLECTION: Collect specimen prior to antimicrobial therapy. Clean the surface of the lesion with saline, and blot dry. Gently remove any crusts, and discard. Abrade superficially until slight bleeding occurs. Wipe away the first few drops of blood. Apply gentle pressure at lesion base, touching clear exudate in ulcer base with a glass slide. Place coverslip and transport immediately to laboratory. TRANSPORT CONDITIONS: Room Temperature (25±5°C): 15 minutes Refrigerated (5±3°C): Unacceptable Frozen (-15±5°C): Unacceptable |
| TURNAROUND TIME (TAT) |
30 minutes |
| REFERENCE RANGE | Negative |
| TEST METHOD | Microscopy |
| CPT CODES |
87210 |